Journal article
Development of a hepatic cryoinjury model to study liver regeneration
Marcos Sande-Melon, David Bergemann, Miriam Fernández-Lajarín, Juan Manuel González-Rosa, Andrew G Cox
Development | The Company of Biologists | Published : 2024
DOI: 10.1242/dev.203124
Abstract
The liver is a remarkable organ that can regenerate in response to injury. Depending on the extent of injury, the liver can undergo compensatory hyperplasia or fibrosis. Despite decades of research, the molecular mechanisms underlying these processes are poorly understood. Here, we developed a new model to study liver regeneration based on cryoinjury. To visualise liver regeneration at cellular resolution, we adapted the CUBIC tissue-clearing approach. Hepatic cryoinjury induced a localised necrotic and apoptotic lesion characterised by inflammation and infiltration of innate immune cells. After this initial phase, we observed fibrosis, which resolved as regeneration re-established homeostas..
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Awarded by NHLBI NIH HHS
Awarded by American Heart Association
Awarded by NIH HHS
Awarded by Australian Research Council
Awarded by National Health and Medical Research Council
Funding Acknowledgements
M.S.-M. is supported by the Early Postdoc Mobility fellowship from the Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (SNSF). D.B. received support from a Wallonie-Bruselles International Postdoctoral Fellowship. J.M.G.-R. is supported by the National Institutes of Health (1R01HL164749-01), the American Heart Association (19CDA34660207), the Corrigan Minehan Foundation (SPARK Award) and the Hassenfeld Foundation (Hassenfeld Research Scholar Award) . A.G.C. is supported by an National Health and Medical Research Council Investigator Grant (GNT1176650) and an Australian Research Council Discovery Project Grant (DP200102693). Open access funding provided by the University of Melbourne. Deposited in PMC for immediate release.